![]() Everything is under your control if you can just figure out the best way to make what you want happen, and the first time or two in a level is practice for clearing it in style. This makes SuperHot VR just about as perfect an arcade game as you could hope to play. Death is quick and easy, and like the red crystal bad guys you go down with a single hit. The bad guys are very good at flanking tactics, and it’s far too easy to focus on one enemy only to hear the familiar shattering sound of your own defeat by a bullet you weren’t paying attention to. It will all work out fine if you follow the classic shooter advice of “Don’t be where the bullets are”, but even when you’ve got all the time in the world to scope out and react to the situation that’s not as easy as it sounds. One level might only have a single ashtray to fling at an enemy, causing him to drop his weapon on death so you can grab it out of the air and return fire, but you know there’s major trouble ahead when an area opens up with a couple of pistols, an uzi, and a shotgun or two within arm’s reach. A well-placed bullet or two and the area is cleared of anything living except me.Įvery level is its own unique scene and starts with a few weapons within easy reach. ![]() I use the last knife to slice the second enemy’s bullet from the air, then duck enemy #3’s shots to reach for the gun the first dead goon dropped. Practice makes perfect, though, and he breaks into pieces as the knife connects, but both he and the third enemy have had time to opened fire. The second enemy is on a balcony to the left and I toss another knife his way, but my track record for the longer throw isn’t quite so good. There are two other knives nearby so, while enemy number two is falling apart, I grab them both. I punch the first one, grab his knife, and throw it at the second enemy. There are four enemies to deal with, with the goon wielding a knife within reach. Total elapsed time- 4 seconds.Īnother level also opens with knives but, seeing as it’s midway into the string of levels that make up a section, it starts right in on the action. Staring at it for a second causes the pyramid to grow slightly, triggering the next level’s start. The familiar satisfying sounds of shattering glass kick in, red shards from the broken bad guys fly into my field of vision, and a black pyramid appears in the center of my vision. I’ve been here before because later sections keep killing me, so I reach out with both hands, grab the knives, and sweep my arms to the side without bothering to look. Two knives hang in the air in front of me, waiting to be grabbed before the level draws itself in. As it turns out, that’s more than enough to commit brutal acts of total badassery. ![]() ![]() What was an FPS in its original incarnation is now an all-new game built as a VR shooting gallery, and you no longer move through the full environment but are constrained within the small, safe area you’ve set up for the headset. That’s not entirely true, of course, but everything is going slowly enough that you’ve got all the time in the world to consider your next move before exploding into a flurry of violence. SuperHot VR doesn’t do anything that hasn’t been done already, but does it so well and with such a great sense of style that it’s easily one of the best pure gaming VR experiences available.īy now the central hook of SuperHot should be familiar- time only moves when you do. ![]() Instead it seems we’ll be seeing a slowly-improving series of games that add to and expand on what’s come before, with the occasional title that takes what we already know and goes nuts with the presentation. Everyone is waiting for a VR killer app, but it’s looking more and more likely that’s not going to happen. ![]()
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![]() ![]() We determined that the practical range of the expression vector length is in between 450 and 950 bp, where it reaches up to 62% efficiency of converting input linear double-stranded DNA (dsDNA) into the circular expression vectors the method could be also used with lower efficiency for somewhat longer fragments. Moreover, our method takes advantage of this difference in properties by using exonuclease to digest unreacted or misreacted linear DNA fragments, thereby purifying the circular DNA. Linear DNA lacks such stability, which is a significant barrier to utilizing it for gene delivery. ![]() Circularized DNA is resistant to exonuclease degradation in the cytoplasm 14. We have developed an efficient fully synthetic method and protocol that enables the production of circularized DNA containing expression elements ready for transfection in as little as 3 h, thereby eliminating the bacterial cloning steps. However, the preparation of plasmids is labor intensive and time consuming process that typically takes 2 days 2 (reference typical bacterial cloning steps in Supplementary Note 1). The approach (I), gene editing using plasmid transfection for transient expression is commonly used due to its conceptual simplicity, ease of handling, and the stability of plasmids. ![]() The choice of delivery method will depend on the specific requirements and the researcher’s preferences, which can include electroporation 9, 10, lipofection 11, direct physical transfection 12, vector delivery on polymer particles and microcarriers 13, and modalities of the aforementioned methods. A variety of delivery methods from the aforementioned gene editing constructs are currently being used. The three most customary approaches that facilitate gene editing when delivered into cells are: (I) single or multiple plasmids 2 encoding Cas9 and other supplemental proteins and implementations of guide RNA suitable for traditional CRISPR/Cas9, base editing, prime editing or other methods 3, 4, 5, 6 (II) Cas9 mRNA and guide RNA 7 (III) Cas9 protein complexed with guide RNAs 8. Although we will discuss the specific implementation for gene editing and compare our method with some existing preparation techniques, the goal of this paper is to present a new approach to preparing the expression vectors in a laboratory environment-and not a more efficient gene editing method as such. However, since the principle behind this method is generic, this method can be useful for a variety of purposes and applications that require the expression of RNAs and proteins. As a demonstration, we will describe the use of our method in the gene editing application context. In this study, we present a new and efficient preparation method. Many techniques exist for the preparation and delivery of RNAs and proteins for transient expression in cell cultures 1. The method’s speed of preparation, low cost, and ease of use will make it a useful tool in applications requiring the expression of short RNAs and proteins. ![]() As proof of the principle, we applied Circular Vector expressing engineered prime editing guide RNA (epegRNA) in cell culture, and demonstrated matching and even exceeding performance of this method as compared to guides expressed by plasmids. The protocol describes how to take a linear double-stranded DNA fragment and efficiently circularize and purify this DNA fragment with minimal hands-on time. We have developed an efficient fully synthetic method and protocol that enables the production of circularized DNA containing expression elements ready for transfection in as little as 3 hours, thereby eliminating the bacterial cloning steps. While the expression plasmids can be designed and ordered from the contract manufacturers, the cost may be prohibitive when a large number of plasmids is required. The preparation of plasmids typically involves a laborious process of bacterial cloning, validation, and purification. DNA plasmids are an essential tool for delivery and expression of RNAs and proteins in cell culture experiments. ![]() ![]() The proposed landing site for Perseverance, Jezero Crater, looks like a former lake basin fed by a dried-out river, a happy hunting ground for a rover prowling for the desiccated remains of early inhabitants. Once the Perseverance team confirmed the first sample tube was on the surface, they positioned the WATSON camera located at the end of the rover’s robotic arm to peer beneath the rover. 21, 2022, the 653rd Martian day, or sol, of the mission. The rover is searching for past life on Mars and collecting soil and rock samples for future. NASA’s Perseverance rover deposited the first of several samples onto the Martian surface on Dec. 18, 2021, it will seek signs of past microbial life and characterize the planets clima. The rationale is simple: If Mars ever had life, the dead will surely outnumber the living, and are therefore more likely to be found. Perseverance is a NASA Mars rover that landed on Feb. When NASAs Mars 2020 rover lands on the Red Planet on Feb. These could contain clues to organisms that pitched and swirled in long-vanished seas. Nasas Perseverance rover is sitting on the surface of Mars after a journey from Earth of almost seven months. Instead, Perseverance will trundle around Mars’ gaunt landscapes searching for sediments. Unlike the Viking landers, the new Perseverance rover isn’t looking for chemical signs of metabolism. So, NASA is now taking a different approach in its hunt for microbes. ![]() This wishy-washy answer was frustratingly ambiguous, especially for a $1 billion experiment. Was there life on Mars? NASA concluded: Probably not. But with further analysis, early optimism soured. The landers initially sent back data that seemed consistent with bacteria-like organisms in the soil. The artwork is etched onto a metal plate attached to the deck of the Mars Perseverance rover. This image depicts all of the rovers which have driven on Mars, as well as the Mars helicopter. NASA's Perseverance rover landed on the Red Planet in February 2021.The hunt for red planet residents began close to four decades ago, when the agency sent two spacecraft - the Viking landers - to Mars. The names of the five rovers are: Sojourner, Spirit and Opportunity, Curiosity, and Perseverance. “But at the same time, the most common way to create these kinds of landforms would be a river.” The Perseverance Mars rover is part of NASA’s Mars Exploration Program, a long-term effort of robotic exploration of the Red Planet. "These layers are anomalously tall for rivers on Earth,” Ives said. Scientists think these tall layers might have been formed by a powerful river, but they're also exploring other explanations. But there is debate on if this river was more like the Mississippi River, which winds snakelike across the landscape, or a braided river like Nebraska’s Platte, which forms small islands of sediment called sandbars.Ī second location, dubbed "Pinestand," is a hill with sedimentary layers that curve toward the sky - some as high as 66 feet. One location in the Jezero Crater, nicknamed “Skrinkle Haven,” has curved layers that scientists determined were formed by powerfully flowing water. ![]() "The more powerful the flow of water, the more easily it’s able to move larger pieces of material." ![]() ![]() “Those indicate a high-energy river that’s truckin’ and carrying a lot of debris," Libby Ives, a postdoctoral researcher at NASA’s Jet Propulsion Laboratory, said in a news release. MORE IMAGES: UAE releases stunning images of Deimos, Mars' smaller moon, that could challenge a long-standing theory ![]() ![]() The elves differ from the extremely tiny figures that are typically depicted as assistants to Santa Claus in popular American mythology. “‘Oh heavens! The elves have stolen the real baby and left this thing instead.’” The more elaborate stories cropped up in the folklore of the 16th and 17th centuries and have ripened with age. The older texts do not divulge much about what the elves do they mainly focus on the activities of the gods. Jacqueline Simpson, a visiting professor at the University of Chichester’s Sussex Centre for Folklore, Fairy Tales, and Fantasy, in England, says references to the word alfar, or “elf,” first appeared in the Icelandic record in Viking-era poems that date back to around 1000 A.D. “But to have people through hundreds of years talking about the same things, it’s beyond one or two crazy ladies. “If this was just one crazy lady talking about invisible friends, it’s really easy to laugh about that,” Jónsdóttir says. That poll is fairly consistent with other findings and with qualitative fieldwork, according to an academic paper published in 2000 titled “The Elves’ Point of View,” by Valdimar Hafstein, who now is a folkloristics professor at the University of Iceland. In one 1998 survey, 54.4 percent of Icelanders said they believed in the existence of them. Though Jónsdóttir’s belief in elves may sound extreme, it is fairly common for Icelanders to at least entertain the possibility of elves’ existence. “So, if one of them is destroyed, it’s, uh, well, it’s not a good thing.”Ī view from Gálgahraun lava field in autumn (Ragnhildur Jónsdóttir) “This elf church is connected by light energy to other churches, other places,” Jónsdóttir says. If a road is completely necessary, the elves will generally move out of the way, but if it is deemed superfluous, a possibility at Gálgahraun, “very bad things” might happen. “I mean, there are thousands or millions of rocks in this lava field,” she says, “but we both went to the same rock or cliff and talked about an elf church.” She knows about the elf church because she can see it, she says, and also sense its energy, a sensation many Icelanders are familiar with. Both she and another seer visited the field separately and came to the same conclusion about the spot. One of the many oddly shaped rocks at the lava field houses “a very important elf church,” which lies directly in the path of one of the roads, according to Jónsdóttir. Jónsdóttir, a greying and spectacled seer who also operates an “elf garden” in nearby Hafnarfjörður, believes that the field is highly populated by elves, huldufolk (“hidden people”), and dwarves, many of whom, she says, have recently fled the area while the matter is settled. At least a few believe it will displace certain supernatural forces that dwell within the hallowed volcanic rubble, and fear the potentially dark consequences that come with such a disturbance. ![]() Not all of the arguments against the development are so straightforward. ![]() One of Iceland’s most famous painters, Jóhannes Sveinsson Kjarval, once worked on his canvases there, perhaps magnetized by the charm of the terrain’s craggy natural relics. According to protester Ragnhildur Jónsdóttir, the thoroughfares would destroy some of the “amazingly beautiful lava formations” and spoil a habitat where birds flock and small plants flourish. ![]() ![]() The Hraunavinir, or Friends of the Lava, believe that any benefits from a project that snakes through Gálgahraun are cancelled out by its cultural and environmental costs. The project, led by the Icelandic Road and Coastal Administration and the nearby municipality of Garðabær, will provide a more direct route to and from the tip of the Álftanes peninsula, where the rustic, red-tiled compound of the country’s president and an eponymous hamlet of 2,600 people stand. At the edge of the ancient Gálgahraun lava field, about a 10-minute drive outside Iceland’s capital city of Reykjavík, a small group of local environmentalists has made camp among the gnarled volcanic rock, wild moss, and browning grass to protest a new road development that will slice the bucolic landscape into four sections and place a traffic circle in its core. ![]() ![]()
![]() ![]() No mass flows across the system boundary.Ī piston-cylinder arrangement is the most common closed system. The system boundary usually encloses a device.Įxamples of open systems are hair dryers, boilers or turbines.Ī closed system is often also called a control volume.Ī closed system consists of a fixed amount of mass enclosed by the system boundary. In the case of open systems, mass can flow across the system boundary, in and out of the system. We distinguish between open and closed systems. The system boundary separates the system from the environment or the surroundings.Įnergy in the form of heat can cross the system boundary and the system can perform work or work can be done on the system. This means anything is either inside or outside of the system. The boundaries of this space is called the system boundary. The system is a properly defined region in space. 7.3 The significance of entropy generationĪn important step in any analysis is to define the system we want to study.7.1.3 Heat transfer over a temperature difference.6.5.2 Reversible processes with heat transfer.5.4 Calculating heat transfer using specific heats. ![]() 5.2 The first law for steady state, open systems.3.2.4 Process with Spring Restrained Piston.2.4.3 Temperature or pressure and quality. ![]() ![]() Fans of Strawberry Cough effects find its Haze parent a top pick. If seeking out similar cultivars, look for ones with Strawberry names and strong sativa genes. What strain is similar to the Strawberry Cough marijuana strain? A fifth of its heritage is indica, adding an easy-going vibe to the high. Is Strawberry Cough indica or sativa? Sporting 80% sativa and 20% indica genes, it delivers a burst of happy energy associated with the tropical weed family. Is the Strawberry Cough strain indica or sativa? Let’s finish by answering the common questions about the Strawberry Cough strain. Outdoor specimens are harvest-ready in mid-October and carry 15 ounces of sticky flower each. ![]() Crops require ten weeks to flower indoors and yield over 17 ounces per square meter of your grow room. Tight orange nugs emerge in early August or once you flip the lights. Frequent pruning and good ventilation encourage light penetration and prevent bud rot. The Strawberry Cough weed is resistant to mold, mildew, and most pests, but its foliage gets bushy. Provide fertilizer in moderation to avoid nutrient burn, and employ strong LED lights to encourage bud production. Water your crops regularly and keep them in a ventilated spot. Temperatures of 80–85☏ and relative humidity under 45% further promote vegetative growth. ![]() ![]() It has a sturdy root zone and develops quickly in sandy soils, and this step assists development. They’re easy to accommodate indoors, while outdoor cultivation requires a steady and balmy climate.ĭon’t skip the seed’s germinating process with this cultivar. These crops grow tall, prefer soil, and produce high yields. Strawberry Cough strain and intermediate cultivators go hand in hand. ![]() ![]() If that’s the fortunate case, carefully read the blog’s review policy and make sure that you follow the directions to a T. First, check that the book blog you’re querying is open to review requests. What now? Here are some tips as you go about getting your book reviews: Let’s say that you’re an author, and you’ve found a couple of book blogs that would be perfect fits to review your book. If you’re wondering how to approach a book blog for a review request, please read on. In that case, you’ll be glad to know that most of the book blogs in our directory are open to review requests and accept indie books! We expressly designed this page (and our book marketing platform, Reedsy Discovery) to be useful to indie book authors who need book reviews. If you’re an aspiring author, you might see a book blog more as a book review blog: a place where you can get your yet-to-be published book reviewed. Take a walk around, use the filters to narrow down your search to blogs in your preferred genre, and feel free to bookmark this page and come back, as we do update it regularly with more of the best book blogs out there. Well, good news - we built this directory of the 200 of the best book blogs to satiate your thirst. If you’re a voracious reader, you might think of a book blog as an oasis in the middle of the desert: a place on the Internet that brims with talk about books, books, and more books. I never compare books to others when rating them. I don’t review the books in the true definition of the word I mostly write about how I felt during and after finishing it.2,000 p/month Preferred contact methodSocial media Accepts indie books?Yes Visit blogĢ … 17 Next › Last » So you want to find a book blog? ![]() I consider anything 3 + stars as a good book and a lot of the time 5 stars just isn’t enough. I don’t discriminate, I will literally read anything and everything as long as it has romance, angst or humor in it. I named him after a character by one of my favorite authors Kristen Ashley.ĭepending on how busy my life gets I can read anything between 3 – 4 books a week. ![]() I can safely say it was the BEST present EVER!! I have since discovered some of the most amazing authors I would never have known about if it wasn’t for my Lawson (who yes is my kindle). That is until last year when I got a kindle for my birthday. I’ve always had a book in my hand no matter where I was. I have loved reading since I was 5 years old. I never go anywhere without my Kindle and reading to me is not a hobby, it’s an important part of who I am and if you want to see a huge smile on my face, just ask me to discuss or recommend one of my favourite books! The smile will literally blind you □ Since then I’ve been fortunate to have read some amazing books, expanded my reading genres, forged some great friendships and every day I thank the wonderful authors for bringing their books into my life. The experience I felt reading these books was something I knew I wanted to feel time and time again. The authors had written stories that spoke to me and touched me in a way nothing before them had. I became so consumed by these books and the characters that I spent the whole weekend immersed in these stories and could not put them down. The books were Thoughtless and Effortless by S C Stephens (Kellan Kyle – swoon) and Beautiful Disaster by Jamie McGuire. I’ve always loved reading, but it wasn’t until a long weekend in June 2011 when, on a book researching blitz, I discovered three books that would turn reading into an addiction as well as a love. ![]() ![]() ![]() I do not believe helping people and treating them nice will spoil them, I certainly don't think that coming to Mexico and staying aloof from the locals so they don't get spoiled is being apart of their community. He routinely remains aloof from the legislative details (some would say he just got rolled by Nancy Pelosi) and then proceeds to speak as if the actual bill matches his grand words and promises when it is obvious to all that it does not.Ĭoyote Blog » Blog Archive » Feature Not a Bug 2010 IPCC To Investigate Barwell Deaths « POLICE INSPECTOR BLOG Inspector Gadget 2009 That is why it is natural that cases like that of the Pilkingtons will be ideal springboards to attack modern day reactive policing which is detached and seemingly aloof from the public they should be serving. Definition of aloof in an aloof manner 'the local gentry and professional classes had held aloof for the school had accepted their sons readily enough' remote in manner 'stood apart with aloof dignity' 'a distant smile' 'he was upstage with strangers' Anagrams of aloof LOOFA Thanks for visiting The Crossword Solver 'aloof'. Why Businesses Are Fed Up With Their Law Firms Richard L. Law firms, by contrast, often hold themselves aloof from the stark realities their clients face. In the US, scientists have been aloof from the political process. I do not believe that scientists were aloof from the political process and find it odd that scientists should be considered policymakers.Ī Public Policy Scientific Consensus? 2009 We cannot afford to remain aloof from the constitutional debate. In short, to grow our intellectual capital and to ensure its steady flow to the business community, we can no longer afford to remain aloof from the economic and business environment.īusiness Education in Canada: A Blueprint For Change 1996 The Romance of China: Excursions to China in U.S. Why did Dunn remain aloof from the expatriate society in Macao for so many years when other traders partook of it regularly? preposition obsolete away from clear ofįrom WordNet 3.0 Copyright 2006 by Princeton University. ![]() adjective Reserved and remote either physically or emotionally distant.preposition obsolete Away from clear from.įrom Wiktionary, Creative Commons Attribution/Share-Alike License.adverb At or from a distance, but within view, or at a small distance apart away.At or to a distance from away or apart from.įrom the GNU version of the Collaborative International Dictionary of English.At a distance, but within view intentionally remaining apart, literally or figuratively withdrawn. /luf/ adjective remote in manner stood apart with aloof dignity synonyms: distant, remote, upstage reserved marked by.adjective Emotionally reserved or indifferent.From The American Heritage® Dictionary of the English Language, 5th Edition. ![]() ![]() Don't forget to have that bin folder to be added to your path. Next, placed it in my /home/user/bin folder, chmod +x /home/user/bin/remove-usb.sh. A sudden loss of power can cause many different kinds of failure in a hard disk drive. There is no well-known way to 'reset' them. Just plug power in, turn it on, and SMART starts watching and logging to it's own NV registers. The values are generated and accumulated in the hard drive itself. Udisks -unmount /dev/$(echo $DEVICE)1 & udisks -detach /dev/$DEVICE SMART is a firmware feature on hard drive, not any operating system or software. Here it is: #!/bin/shĭEVICE=$( udisksctl status | grep -i sandisk | xargs echo | cut -d' ' -f6 ) Next, I've put together the script remove-usb.sh. Of course for you, you'd have to use udisksctl status | grep -i wd | xargs echo and count which element is your block id is, and then use appropriate number on cut -d' ' -fx Here we can use cut command to extract it like so: udisksctl status | grep -i sandisk | xargs echo | cut -d' ' -f6 What would be the output of this command : udisksctl status | grep -i sandisk | xargs echo ? It would be this: SanDisk Cruzer Glide 1.27 2005425573077FA0AA67 sdd, or in other words information about the usb, delimited by spaces, with the device id being last argument, or the 6th one. We'd need to let the shell know the block id of out external hdd or usb. However, at 45,000 hours of use I would be backing up your critical files and expecting drive failure sooner rather than later. What if we could write a script to do the safe-ejecting for us ? This will tell you if there are any slow or unresponsive sectors on the disk surface, which are a far more accurate predictor of drive failure than the disk temperature. Share Improve this answer Follow answered at 15:03 Chris H 19 1 1 This is incorrect. I have one drive currently with a power on count of 2,401 and 36,615 hours that is fine and another that has a power on count of 95 and 17,442 total hours that has failed. Now, here's something I've done purely for fun, but you could find it useful and adapt to your own needs. Unfortunately powering on and off a drive does not wear it out faster or slower. TIP: you can use udisksctl status | grep -i wd to show only information for the external hard drive. With the one-liner I use, I've noticed that the device id is preserved, i.e, if i eject the usd and reconnect it, it still gives the device sdc name. So in my case, I would eject the usb with udisks -unmount /dev/sdc1 & udisks -detach /dev/sdc or alternatively with what Fabby suggested. Each time you pugin a usb device it will be identified with different letters, sdb, sdc,sdf and so forth, while sda is always your hard-drive. The output of the commands is the following:Īs you can see both report that my usb drive is identified as /dev/sdc. For instance, I have a Sandisk Cruiser usb drive connected right now. ![]() You can use lsblk and identify it by mount-point and size on the other hand, you can use udisksctl status. Basically, i use udisks -unmount /dev/sdb1 & udisks -detach /dev/sdb where sdb1 is the mount-point and sdb is the actual disk. I have answered related question some time ago, when just started out with ubuntu, so I suggest you refer to it as well. Then you can use either udisks or udisksctl to unmount and actually power off the device. If it's not, continue with the steps below.So far I haven't found a simple alternative to "safely eject" in the file manager, so this is my justification for using command line here.įirst, you'd need to find out your external hard-drives device id or block id. Under the "The following states are available on this system" section, if Hibernate appears, then the feature is enabled.Type the following command to confirm the hibernation status and press Enter: powercfg /availablesleepstates.4 Once a drive has surpassed the 43,800 hour mark, it may no longer be classed as in 'perfect condition'. Search for Command Prompt, right-click the top result, and select the Run as administrator option. Power-on hours is intended to indicate a remaining lifetime prediction for hard drives and solid state drives, generally, 'the total expected life-time of a hard disk is 5 years' 3 or 43,800 hours of constant use. ![]() To check and enable hibernation on Windows 11, use these steps: When using hibernation, the resume process is slower than sleep, but it helps you conserve more battery. The feature works by saving the data in memory onto the hard drive, allowing you completely shut down and resume where you left off without wasting power. On Windows 11, hibernation is a feature that can help preserve battery life when you are not using the device. Source: Windows Central (Image credit: Source: Windows Central)Īlternatively, you can go to Settings > Apps & features, select the app, click the menu (three-dotted) button, click on Advanced options, and then change the "Background apps permissions" setting. ![]() |